Family: Master of Sempill Robert Sempill / Barbara Preston (F12081)
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 | 3rd Lord Sempill Robert Sempill (1501-1573)
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 | Lady Isabel Hamilton (1508-1546)
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| Archibald Preston (1515-1594)
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| Giles Sempill (1515-1609) |
| Master of Sempill Robert Sempill (1522-1569)
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| Barbara Preston (1540-1631)
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| Fourth Lord of Sempill Robert Sempill (1564-1611)  |